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Mouse Phenotypes: Generation and Analysis of Mutants, Second Edition: A Laboratory Manual by Papaioannou, Virginia E.

Mouse Phenotypes: Generation and Analysis of Mutants, Second Edition: A Laboratory Manual

by Virginia E. Papaioannou , Richard R. Behringer
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Book cover type: Paperback
  • ISBN13: 9781621824183
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Cold Spring Harbor Laboratory Press
  • Publisher Imprint: Cold Spring Harbor Laboratory Press
  • Publication Date:
  • Pages: 306
  • Original Price: USD 95.0
  • Language: English
  • Edition: 2 LAB
  • Item Weight: 817 grams
  • BISAC Subject(s): Animals / Mammals

In this postgenome era, the large number of mouse mutations available is revolutionizing biomedical research. Large-scale mutagenesis projects have resulted in the modification of thousands of mouse genes and, more recently, with CRISPR-Cas gene editing, generation of custom mutations is easier than ever. The interpretation of mutant phenotypes is critically important for understanding how changes in the genome result in changes in the phenotype. Complex phenotypes resulting from gene manipulation or spontaneous mutation, however, provide a huge analytic challenge. Where does one start with the analysis? How does one dissect the phenotype and begin to understand the molecular and developmental consequences of the genotype? How does one make the best use of the valuable resource that you have developed?

Mouse Phenotypes: A Handbook of Mutation Analysis, Second Edition, provides a road map for such analysis. Advice and practical guidance are provided, starting with the planning stages of gene targeting, CRISPR-Cas gene editing, or mutagenesis experiments, through the complete analysis of complex phenotypes, including tips on breeding strategies, studying dominant mutations, and characterizing a mutant mouse with no apparent phenotypic abnormalities. A systematic approach to the analysis, complemented with simple protocols, will help users make a comprehensive assessment of a particular phenotype resulting from a mutation and determine the timing and likely causes of specific developmental failures. Investigators using the laboratory mouse in genetic studies designed to understand mammalian biology will benefit from the authors' experience and advice in analyzing mutant phenotypes.

Behringer, Richard R.: - Richard R. Behringer is Professor in the Department of Genetics, Division of Basic Science Research at The University of Texas MD Anderson Cancer Center

Papaioannou, Virginia E.: - Virginia E. Papaioannou is a Special Lecturer in Genetics and Development and Professor Emerita of Genetics and Development at Columbia University

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