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Nanoscale Imaging and Characterisation of Amyloid-β by Claire Louisa Tinker-Mill

Nanoscale Imaging and Characterisation of Amyloid-β

by Claire Louisa Tinker-Mill
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Book cover type: Hardcover
  • ISBN13: 9783319395333
  • Binding: Hardcover
  • Subject: N/A
  • Publisher: Springer
  • Publisher Imprint: Springer
  • Publication Date:
  • Pages: 149
  • Original Price: EUR 99.99
  • Language: English
  • Edition: 2016
  • Item Weight: 418 grams
  • BISAC Subject(s): Spectroscopy & Spectrum Analysis, Life Sciences / Molecular Biology, and Mechanics / Fluids

From the Back Cover
This thesis presents a method for reliably and robustly producing samples of amyloid-β (Aβ) by capturing them at various stages of aggregation, as well as the results of subsequent imaging with various atomic force microscopy (AFM) methods, all of which add value to the data gathered by collecting information on the peptide's nanomechanical, elastic, thermal or spectroscopical properties.
Amyloid-β (Aβ) undergoes a hierarchy of aggregation following a structural transition, making it an ideal subject of study using scanning probe microscopy (SPM), dynamic light scattering (DLS) and other physical techniques. By imaging samples of Aβ with Ultrasonic Force Microscopy, a detailed substructure to the morphology is revealed, which correlates well with the most advanced cryo-EM work. Early stage work in the area of thermal and spectroscopical AFM is also presented, and indicates the promise these techniques may hold for imaging sensitive and complex biological materials. This thesis demonstrates that physical techniques can be highly complementary when studying the aggregation of amyloid peptides, and allow the detection of subtle differences in their aggregation processes.

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