Nanotechnology for Nucleic Acid Delivery: Methods and Protocols
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1. Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for Transfection
S�ren Reinhard and Ernst Wagner
2. Synthesis of Bioreducible Polycations with Controlled Topologies
Ye-Zi You, Jun-Jie Yan, Fei Yu, Zhi-Qiang Yu, and David Oupick�
3. Histidine-Rich Cationic Cell Penetrating Peptides for Plasmid DNA and siRNA Delivery
Antoine Kichler, A. James Mason, Arnaud Marquette, and Burkhard Bechinger
4. Covalent Fluorophore Labeling of Oligonucleotides and Generation of Other Oligonucleotide Bioconjugates
Cornelia Lorenzer and Johannes Winkler
5. Synthetic Oligonucleotides in SPECT/CT In Vivo Imaging: Chemical Modifications, In111 Complex Formation, Incorporation into Drug Delivery Systems
Maxim Antopolsky
6. Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids
Wolfgang R�dl, Alexander Taschauer, David Schaffert, Ernst Wagner, and Manfred Ogris
7. Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery
Manon Ripoll, Patrick Neuberg, Jean-Serge Remy, and Antoine Kichler
8. Lipids for Nucleic Acid Delivery: Cationic or Neutral Lipoplexes, Synthesis, and Particle Formation
Michel Bessodes, Helene Dhotel, and Nathalie Mignet
9. Preparation, Characterization, and In Vitro Evaluation of Lipidoid-Polymer Hybrid Nanoparticles for siRNA Delivery to the Cytosol
Kaushik Thanki, Xianghui Zeng, and Camilla Foged
10. Layer-By-Layer Assembled Nanoparticles for siRNA Delivery
Michaela Guter and Miriam Breunig
11. Layer-By-Layer Film Engineering for Sequential Gene Delivery
Lingxiao Xie, Yi Zou, Sean Carroll, Maria Muniz, and Guangzhao Mao
12. Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles
Angela K. Pannier, Tyler Kozisek, and Tatiana Segura
13. In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films
Yi Zou, Lei Wan, Jenifer Blacklock, Lingxiao Xie, Sean Carroll, David Oupicky, and Guangzhao Mao
14. Lyophilization of Synthetic Gene Carriers
Julia Christina Kasper, Sarah Hedtrich, and Wolfgang Friess
15. Firefly Luciferase-Based Reporter Gene Assay for Investigating Nanoparticle-Mediated Nucleic Acid Delivery
Katharina M�ller, Manfred Ogris, and Haider Sami
16. Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles
Heleen Dewitte, Silke Roovers, Stefaan C. De Smedt, and• Author(s): Clear | James • Publisher: Penguin • Publisher Imprint: Penguin Random House • Subject: General Books
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