Opracowanie konstrukcji genu reporterowego do lokalizacji subkomórkowej

Name: Opracowanie konstrukcji genu reporterowego do lokalizacji subkomórkowej
Brand: Wydawnictwo Nasza Wiedza
SKU: 9786630019759
Price: 7340.0 INR
Availability: InStock

by Biruk Abrha

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ISBN13: 9786630019759
Binding: Paperback
Subject: N/A
Publisher: Wydawnictwo Nasza Wiedza
Publisher Imprint: Wydawnictwo Nasza Wiedza
Publication Date: May 31, 2026
Pages: 88
Original Price: GBP 58.0
Language: Polish
Edition: N/A
Item Weight: 127 grams
BISAC Subject(s): Life Sciences / Genetics & Genomics

About The Book

Pirofosfohydrolaza RNA (RppH) inicjuje degradację transkrypt�w poprzez usuwanie pirofosforanu z 5'-końca mRNA, co umożliwia wiązanie się RNazy E/RNazy J w bakteriach. W kom�rkach C. reinhardtii występuje domniemana pirofosforylaza RNA, a kilka dowod�w sugeruje, że bialko to bierze udzial w degradacji mRNA w chloroplastach. W celu określenia lokalizacji bialka w kom�rkach Chlamydomonas, koniec 5' genu rppH zostal oznaczony za pomocą zoptymalizowanego pod kątem kodon�w bialka zielonej fluorescencji (GFP) i wprowadzony do kom�rek Chlamydomonas. GFP (Zsgreen 1) zostalo zoptymalizowane pod kątem kodon�w przy użyciu bazy danych wykorzystania kodon�w. Ramka odczytu zoptymalizowanego syntetycznego GFP zostala dostosowana i sklonowana do wektora pBluescript-5'rppH SK+. Fragment genu 5'rppH-GFP zostal następnie sklonowany do trzech wektor�w. Wszystkie konstrukty zostaly zweryfikowane. Transformanty zostaly przebadane pod kątem obecności chimerycznego genu GFP za pomocą PCR. Eksperymenty przesiewowe doprowadzily nas do wyboru jednego transformanta, kt�ry m�glby zostac wykorzystany do dalszych prac. Ponadto, chociaż nie sprawdzono fluorescencji, zoptymalizowane syntetyczne GFP uleglo ekspresji w kom�rkach E. coli (BL21DE3), potwierdzając funkcjonalnośc syntetycznego konstruktu GFP in vivo.

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