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PCR Sequencing Protocols by Ralph Rapley

PCR Sequencing Protocols

by Ralph Rapley
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Book cover type: Paperback
  • ISBN13: 9781489940384
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Springer
  • Publisher Imprint: Humana
  • Publication Date:
  • Pages: 221
  • Original Price: EUR 99.99
  • Language: English
  • Edition: 1996
  • Item Weight: 346 grams
  • BISAC Subject(s): Life Sciences / Cell Biology, Life Sciences / Molecular Biology, and Laboratory Medicine

Advances in bioscience research usually arise as a result of the continu- ing refinement of existing technologies. However, there are a number of occa- sions v rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu- tionary techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech- nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac- tion. This technique, first reported in 1985 by MuUis and his colleagues, pro- vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase.

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