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Platelets and Megakaryocytes: Volume 1: Functional Assays by Jonathan M. Gibbins

Platelets and Megakaryocytes: Volume 1: Functional Assays

by Jonathan M. Gibbins
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Book cover type: Paperback
  • ISBN13: 9781617373565
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Springer
  • Publisher Imprint: Humana
  • Publication Date:
  • Pages: 385
  • Original Price: EUR 109.99
  • Language: English
  • Edition: N/A
  • Item Weight: 592 grams
  • BISAC Subject(s): Hematology, Physiology, and Life Sciences / Molecular Biology

From the Back Cover
Platelets are an essential element of the body's hemostasis system, and yet, through their involvement in thrombosis, are also a major cause of morbidity and mortality. Platelets have a limited lifespan and must be continually manufactured by their precursor, the megakaryocyte. In Platelets and Megakaryocytes, Volume 1: Functional Assays, leading expert researchers-many responsible for seminal discoveries in the field-describe basic and advanced techniques for analyzing platelet and megakaryocyte function in step-by-step detail. The approaches presented for platelet analysis include aggregometry, secretion, arachidonic acid metabolism, procoagulent response, platelet adhesion under static and flow conditions, flow cytometry, and production of microparticles. Techniques presented for megakaryocyte function research include those used to study the development of specialized structures for platelet production, the appearance of platelet-specific surface receptors, and the increase in ploidy. All protocols follow the successful Methods in Molecular Biology(TM) series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Platelets and Megakaryocytes, Volume 2: Perspectives and Techniques offers a state-of-the-art survey of our understanding of these cells, with emphasis on the role of cell signaling in their regulation and function, as well as the use of molecular and post-genomic techniques for their study.
Comprehensive and cutting-edge, the two volumes of Platelets and Megakaryocytes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.

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