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Preparazione, caratterizzazione e valutazione in vitro di scaffold 3D by Paulo A. L. Lima, Gabriela S. Andrade, Luis E. Almeida

Preparazione, caratterizzazione e valutazione in vitro di scaffold 3D

by Paulo A. L. Lima , Gabriela S. Andrade , Luis E. Almeida
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Book cover type: Paperback
  • ISBN13: 9786209379055
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Edizioni Sapienza
  • Publisher Imprint: Edizioni Sapienza
  • Publication Date:
  • Pages: 60
  • Original Price: GBP 34.71
  • Language: Italian
  • Edition: N/A
  • Item Weight: 96 grams
  • BISAC Subject(s): General

Gli scaffold 3D di chitosano (CHI), fibroina (SF) e idrossiapatite (HA) sono biocompatibili e interessanti per applicazioni nell'ingegneria tissutale ossea. Pertanto, l'obiettivo era quello di preparare, caratterizzare e valutare il comportamento in vitro degli scaffold 3D di CHI, CHI/SF, CHI/SF/HA. I biomateriali sono stati prodotti a partire da una soluzione di CHI, CHI/SF e CHI/SF/HA in acido acetico e, dopo la realizzazione dei processi di produzione, sono stati sottoposti a test in vitro con cellule SaOS-2 per 7, 14 e 21 giorni. Gli scaffold sono stati caratterizzati mediante diffrazione dei raggi X, spettroscopia infrarossa con trasformata di Fourier con riflettanza totale attenuata, analisi termogravimetrica, calorimetria differenziale esplorativa, microscopia elettronica a scansione, spettroscopia a energia dispersiva e sottoposti a test per verificare la porosit�, la dimensione dei pori e l'angolo di contatto. La fattibilit� e l'attivit� cellulare sono state valutate mediante riduzione dell'MTT e rilevamento dell'attivit� della fosfatasi alcalina. Gli scaffold 3D sono stati considerati atossici e in grado di promuovere l'adesione, la proliferazione e la differenziazione cellulare, potendo essere utilizzati nella terapia di riparazione dei difetti ossei.

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