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Protein Carbonylation: Principles, Analysis, and Biological Implications by Joaquim Ros

Protein Carbonylation: Principles, Analysis, and Biological Implications

by Joaquim Ros
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Book cover type: Hardcover
  • ISBN13: 9781119074915
  • Binding: Hardcover
  • Subject: N/A
  • Publisher: Wiley
  • Publisher Imprint: Wiley
  • Publication Date:
  • Pages: 416
  • Original Price: USD 202.95
  • Language: English
  • Edition: N/A
  • Item Weight: 617 grams
  • BISAC Subject(s): Spectroscopy & Spectrum Analysis and Life Sciences / Biochemistry

Protein carbonylation has attracted the interest of a great number of laboratories since the pioneering studies at the Earl Stadtman’s lab at NIH started in early 1980s. Since then, detecting protein carbonyls in oxidative stress situations became a highly efficient tool to uncover biomarkers of oxidative damage in normal and altered cell physiology.

In this book, research groups from several areas of interest have contributed to update the knowledge regarding detection, analyses and identification of carbonylated proteins and the sites where these modifications occur.

The scientific community will benefit from these reviews since they deal with specific, detailed technical approaches to study formation and detection of protein carbonyls. Moreover, the biological impact of such modifications in metabolic, physiologic and structural functions and, how these alterations can help understanding the downstream effects on cell function are discussed.

*Oxidative stress occurs in all living organisms and affects proteins and other macromolecules: Protein carbonylation is a measure of oxidative stress in biological systems *Mass spectrometry, fluorescent labelling, antibody based detection, biotinylated protein selection and other methods for detecting protein carbonyls and modification sites in proteins are described *Aging, neurodegenerative diseases, obstructive pulmonary diseases, malaria, cigarette smoke, adipose tissue and its relationship with protein carbonylation *Direct oxidation, glycoxidation and modifications by lipid peroxidation products as protein carbonylation pathways *Emerging methods for characterizing carbonylated protein networks and affected metabolic pathways

Joaquim Ros is Professor at the University of Lleida. From 1995 his research interest has been focused on studying the effect of oxidative stress on proteins in several models (from bacteria to humans) and how this damage affects protein function. He is the head of the Dept. of Basic Medical Sciences, Faculty of Medicine, University of Lleida.

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