Purifying and Culturing Neural Cells: A Laboratory Manual

Name: Purifying and Culturing Neural Cells: A Laboratory Manual
Brand: Cold Spring Harbor Laboratory Press
SKU: 9781936113996
Price: 6294.0 INR
Availability: InStock

by Ben A. Barres , Beth Stevens

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ISBN13: 9781936113996
Binding: Paperback
Subject: N/A
Publisher: Cold Spring Harbor Laboratory Press
Publisher Imprint: Cold Spring Harbor Laboratory Press
Publication Date: November 30, 2013
Pages: 205
Original Price: GBP 56.0
Language: English
Edition: Lab Manual
Item Weight: 704 grams
BISAC Subject(s): Life Sciences / Biochemistry

About The Book

Cell culture systems for specific neural cell types are essential for studies of their development and function. This laboratory manual provides step-by-step protocols for isolating specific cell populations from rodent tissues and culturing them under conditions that closely resemble those in vivo. The contributors describe in detail how to dissect the brain, spinal cord, and other tissues; how to separate cells using mechanical and enzymatic tissue-dissociation strategies; the use of immunopanning and fluorescence-activated cell sorting (FACS) to enrich the target cell population; and the culture conditions that optimize cell viability and growth. Retinal ganglion cells, motor neurons, dorsal root ganglion cells, astrocytes, oligodendrocytes, and Schwann cells are covered, as are vascular cells such as pericytes and endothelial cells. Myelinating co-cultures of neurons and oligodendrocytes are also described. The manual includes detailed recipes for media and reagents, tips for avoiding common pitfalls, and advice for designing new immunopanning protocols using tissues from other sources. Many of the protocols are accompanied by freely accessible online movies that demonstrate critical steps of the procedures. This is an essential laboratory companion for all neurobiologists, from the graduate student level upwards.

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