This systematically designed laboratory handbook elucidates a number of techniques which help students carry out various experiments in the field of biochemistry. The experimental protocols described in this book have been standardized and performed in the authors’ own laboratory.
It is basically intended for the undergraduate and postgraduate students of life sciences (biochemistry, microbiology, biotechnology, plant biotechnology, animal biotechnology, botany and zoology) and engineering (biotechnology and biomedical) for their laboratory courses. The students usually have to refer to many journals and books to find the right procedure for performing experiments, hence this handbook is an attempt to provide them with the frequently used methods in a handy format, including explanations of principles, procedures and interpretations of results of the experiments.
Now, in its second edition, the book introduces ten new experiments in a step-by-step procedural format under In Vitro Enzymatic Anti-oxidant Assays explaining Determination of Nitric Oxide Radical Scavenging Activity, Determination of Catalase Activity, Determination of Laccase Activity and Concentration and Diafiltration.
KEY FEATURES
• Provides a general procedure of the experiments in an easy-to-understand tabulated format.
• Presents the physiological importance of bio-components like amino acids, uric acid, carbohydrates, proteins, etc. in the human body as an added feature.
• Gives information on preparation of laboratory reagents in separate appendices.
• Provides illustrations for better understanding of the experiments.
TARGET AUDIENCE
• B.Sc. / M.Sc. Life sciences (Biochemistry, Microbiology, Biotechnology, Plant Biotechnology, Animal Biotechnology, Botany and Zoology)
• B.Tech (Biotechnology and Biomedical Engineering)
S. SHANMUGAM is Associate Professor,
PG Research, Department of Microbiology and Biotechnology, Presidency College (Autonomous) Chennai. Having more than 20 years of teaching experience and 15 years of research experience, he has published more than 50 peer reviewed research papers in various national and international journals. His area of research interest is Microbial Enzyme Technology.|T. SATHISH KUMAR is Assistant Professor Grade III in the Department of Biotechnology, Kumaraguru College of Technology, Coimbatore. Dr. Kumar has 18 years of teaching experience and 11 years of research experience, and has published more than 40 peer reviewed research papers in various national and international journals. His area of research interest is characterization and medical applications of polyphenols.|K. PANNEER SELVAM is Assistant Professor in the Department of Microbiology, Government Arts College, Mannargudi, Tamil Nadu. He has published several peer reviewed research papers in various national and international journals and conducted many DBT/DST sponsored state level, national and international seminars and training programmes. Dr. Panneer Selvam's areas of research interest include medical microbiology, medical biotechnology and recombinant DNA technology.
