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Résistance Génétique À La Rouille Couronnée by Sliti-Y

Résistance Génétique À La Rouille Couronnée

by Sliti-Y
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Book cover type: Paperback
  • ISBN13: 9783838183589
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Omniscriptum
  • Publisher Imprint: Omniscriptum
  • Publication Date:
  • Pages: 88
  • Original Price: GBP 31.0
  • Language: French
  • Edition: N/A
  • Item Weight: 141 grams
  • BISAC Subject(s): Life Sciences / Genetics & Genomics

La rouille couronnée, causée par Puccinia coronata corda f.sp .avenae. Eriks, est la principale maladie fongique de l'avoine en Tunisie. Dans cette étude, quatre protocoles d'extraction d'ADN ont été testés pour une utilisation ultérieure de l'ADN de qualité dans l'analyse RAPD. La méthode DTT a donné la meilleur qualité et quantité de l'ADN. Vingt-et-une ont pu montrer une variabilité génétique entre les trois génotypes. Elles ont généré un total de 202 bandes, dont 65 sont polymorphes. Les produits d'amplifications varient de 200 à 900 pb de taille et de 4 à 15 en nombre. Les résultats ont montré que JT0 et JT5, ont un indice de similarité faible (0.53) comparé à celui obtenu entre JT5 et Pc68 (0.57) et celui obtenu entre JT0 et Pc68. Le dendrogramme obtenu en utilisant la méthode de Raup-Crik, a regroupé les génotypes en deux groupes. JT0 et Pc68 ont été compilés dans le mème groupe avec un degré significatif de la similitude de 0.7, tandis que JT5 a été isolé seul avec un faible degré de similitude (0.09).L'accession JT0 pourrait héberger le gène de résistance Pc68, tandis que JT5 pourrait héberger un nouveau gène de résistance ou mème un nouveau contrôle génétique.

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