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Retention and Selectivity in Liquid Chromatography: Prediction, Standardisation and Phase Comparisons Volume 57 by R. M. Smith

Retention and Selectivity in Liquid Chromatography: Prediction, Standardisation and Phase Comparisons Volume 57

by R. M. Smith
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Book cover type: Hardcover
  • ISBN13: 9780444815392
  • Binding: Hardcover
  • Subject: N/A
  • Publisher: Elsevier Science
  • Publisher Imprint: Elsevier Science
  • Publication Date:
  • Pages: 456
  • Original Price: GBP 196.99
  • Language: English
  • Edition: N/A
  • Item Weight: 844 grams
  • BISAC Subject(s): Chemistry / Analytic

This book brings together a number of studies which examine the ways in which the retention and selectivity of separations in high-performance liquid chromatography are dependent on the chemical structure of the analytes and the properties of the stationary and mobile phases. Although previous authors have described the optimisation of separations by alteration of the mobile phase, little emphasis has previously been reported of the influence of the structure and properties of the analyte.

The initial chapters describe methods based on retention index group increments and log P increments for the prediction of the retention of analytes and the ways in which these factors are influenced by mobile phases and intramolecular interactions. The values of a wide range of group increments in different eluents are tabulated.

Different scales of retention indices in liquid chromatography are described for the comparison of separations, the identification of analytes and the comparison of stationary phases. Applications of these methods in the pharmaceutical, toxicology, forensic, metabolism, environmental, food and other fields are reviewed. The effects of different mobile phases on the selectivity of the retention indices are reported. A compilation of sources of reported retention index values are given.

Methods for the comparison of stationary phases based on the interactions of different analytes are covered, including lipophilic and polar indices, shape selectivity comparisons, their application to novel stationary phases, and chemometric methods for column comparisons.

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