Spektroskopische Untersuchung an Oxonin und Capriblau GN
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Inhaltsangabe: Für die Untersuchung einer Reihe von biologischen und medizinischen Problemen ist es wünschenswert Gewebeschnitte mit Farbstoffen anzufärben. Das Ziel einer solchen Untersuchung ist es, eine Beziehung von Zellen und Geweben und Ihrer histologischen Struktur herzustellen. Dabei bevorzugt man Farbstoffe, die den Zustand der lebenden Zellen möglichst wenig schädigen und die Zellstrukturen nach Möglichkeit nicht verändern sollen - sogenannte Vitalfarbstoffe. In diesem Zusammenhang sind Vitalfarbstoffe von besonderem Interesse. Zu Ihnen zählen insbesondere Derivate des Phenazins und Phenthiazins wie Neutralrot und Methylenblau. Aber auch Phenoxazine haben in der Histologie Anwendung gefunden. So ermöglicht z.B. Gallocyanin die cytochemische Lokalisation von Nukleinsäuren. Bis heute ist wenig über die physikalischen und chemischen Wechselwirkungen zwischen Farbstoff und biologischem Trägermaterial bekannt. Zweck dieser Untersuchung ist es, durch Messung von Elektronen- und Polarisationsspektren Grundlagenmaterial in die Hand zu bekommen, mit dem man Änderungen der optischen Eigenschaften beim Aufziehen der Farbstoffe auf biologisches Trägermaterial besser beurteilen kann. Es werden zwei einfache Vertreter der Phenoxazinfarbstoffreihe, das 3,7-Diaminophenoxazoniumchlorid (Oxonin) und das 3,7-Bis(dimethylamino)phenoxazoniumnitrat Capriblau (GN) in Ahnlehnung an Abeiten von Kehrmann sowie Kehrmann und Poplawsky spektroskopisch rein dargestellt, um die verschiedenen optischen Eigenschaften zu untersuchen. Inhaltsverzeichnis: Inhaltsverzeichnis: 1.Einleitung und Aufgabenstellung1 2.Spektroskopische Untersuchungen 2.1Elektronenspektren aromatischer Verbindungen3 2.1.1Absorption4 2.1.2Fluoreszenz5 2.2Experimentelle Spektren 2.2.1Normal- und Tieftemperaturspektren von Oxonin und Capriblau GN6 2.2.2Fluoreszenzspektren13 2.3Verhalten der Farbstoffe gegenüber Protonierung 2.3.1Protonierungsgleichgewichte und isosbestische Punkte von Oxonin und Capriblau GN14 2.4Konze
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