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Techniques and Methodological Approaches in Breast Cancer Research by Jose Russo

Techniques and Methodological Approaches in Breast Cancer Research

by Jose Russo
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Book cover type: Paperback
  • ISBN13: 9781493946624
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Springer
  • Publisher Imprint: Springer
  • Publication Date:
  • Pages: 287
  • Original Price: EUR 149.99
  • Language: English
  • Edition: Softcover Repri
  • Item Weight: 435 grams
  • BISAC Subject(s): Oncology / General, Life Sciences / Molecular Biology, and Life Sciences / Biochemistry

From the Back Cover

This book provides a detailed description of the most common techniques used in breast cancer research, with a special emphasis on the study of the normal breast as the basis for understanding the pathological processes in this organ.

The authors have poured their experience in the field of breast cancer research into this book, and described the process of handling and interpreting the complex organ that is the human breast using original data and unique material collected over the years. They have established the concept of normality of the human breast and the morphological parameters to be used for distinguishing the normal from the diseased breast. The quality of the breast tissue obtained from autopsy material vs. core biopsies are carefully detailed and discussed, showing the morphological differences among the specimens according to their collection site. The use of laser capture microdissection and tissue microarray is described in detail and discussed with special references to the problems faced when the normal breast is under study. A chapter dedicated to the use of breast cancer cell lines is crafted around the importance of each cell line and its particularities for in vitro studies. In addition, the authors explore the new concept that cancer originates from two main cell types, basal and luminal cells . Particular attention is given to the study of the phenotypes commonly used for determining cell transformation, either spontaneously or by induced chemical agents. A companion chapter using in vivo models provides the adequate tools for those interested in studying the early events of cancer initiation, progression and metastasis. Special emphasis is given to the analysis of cells either by Flow cytometry and immuno-fluorescence techniques and the great potential of the use of in situ hybridization to study RNA transcripts. The book also features an original analysis of the putative markers of stem cells in breast cancer usingimmuno-cytochemical techniques, their interpretation and correlation with the prognosis of the disease. The last two chapters of the book are engaged in the utilization of RNA and DNA sequencing techniques and their values in understanding the physiological differences in the human breast, as well as the utilization of epigenetic methodologies in the understanding of the normal human breast.

The authors have established the methodological basis for studying the human breast in its normal and pathological conditions providing a unique set of tools and valuable references for researchers pursuing a deeper understanding of the human breast.

Jose Russo, M.D., FACP is a Professor and Senior Member for the Fox Chase Cancer Center. He is also the Director of the Breast Cancer and Environment Research Center at Fox Chase .In addition to his roles at Fox Chase, he is simultaneously an Adjunct Professor of Pathology and Cell Biology at Jefferson Medical College, as well as an Adjunct Professor of Biochemistry at Temple Medical School. His primary research interest includes breast cancer, endocrinology, hormonal and environmental carcinogenesis. He's received several academic and research awards for his outstanding work in the field. He's a member of numerous scientific and professional societies. He has published over 350 scientific papers. He also serves on several editorial boards for several journals including, International Journal of Oncology, Hormone Molecular Biology and Clinical Investigation, and the Journal of Medical Hypothesis and Research. In addition, he's the editor-in-chief of the Journal of Women's Cancer. He's also published several books with Springer in the past. Irma Russo, M.D., FACP, FASCP is Associate Professor at the Fox Chase Cancer Center and the Chief of the Molecular Endocrinology Section oat the Breast Cancer Research Lab. She has over 190 scientific publications.

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