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Technologien zur bakteriellen Genexpression by Zaky, Mahmoud

Technologien zur bakteriellen Genexpression

by Mahmoud Zaky , Merna Abou Selima
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Book cover type: Paperback
  • ISBN13: 9786207760930
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Verlag Unser Wissen
  • Publisher Imprint: Verlag Unser Wissen
  • Publication Date:
  • Pages: 64
  • Original Price: GBP 42.0
  • Language: German
  • Edition: N/A
  • Item Weight: 105 grams
  • BISAC Subject(s): Life Sciences / Microbiology

Die zufällige Integration des IVET-Vektors in das Erregerchromosom erfolgt durch Insertions-Duplikations-Mutagenese, um einen Pool von rekombinanten Erregern zu schaffen (das bedeutet, dass das Gen, in das der Vektor durch homologe Rekombination eingefügt wurde, nicht gestört wird). Die Rekombinanten können mit Hilfe der Antibiotikaresistenz selektiert werden, da sich ein zusätzlicher Marker auf dem integrierten IVET-Konstrukt befindet. Die gepoolten Klone werden dann in die Maus geimpft. Die beiden Haupttypen von IVET-Promotorenfallen-Strategien sind (a) die Komplementierung der auxotrophen Mutation und (b) die Expression der Antibiotikaresistenz. Nur die Bakterien, die den selektiven Marker enthalten, der mit einem Gen fusioniert ist, das im Wirt transkriptionell aktiv ist, können überleben. Nach einer geeigneten Infektionszeit werden Bakterien, die den Marker exprimieren, aus Lungen-, Leber-, Milz- und Mesenterialgewebe isoliert.

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