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The Nuclear Membrane: Methods and Protocols by Yuki Hara

The Nuclear Membrane: Methods and Protocols

by Yuki Hara , Kazunori Kume
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Book cover type: Hardcover
  • ISBN13: 9781071647134
  • Binding: Hardcover
  • Subject: N/A
  • Publisher: Humana
  • Publisher Imprint: Humana
  • Publication Date:
  • Pages: 346
  • Original Price: EUR 199.99
  • Language: English
  • Edition: N/A
  • Item Weight: 840 grams
  • BISAC Subject(s): Life Sciences / Cell Biology

Lipidomic profiling of isolated nuclei from mice and cultured cells.- Profiling plant nuclear envelope composition using subtractive proteomics.- Ultrastructural visualization of the  nuclearenvelope in HeLa cells.- Imaging nuclear envelopes using correlative AFM/fluorescence microscopy.- Fast super-resolution live cell imaging of nuclear membrane-endoplasmic reticulum dynamicsImmunostaining the yeast nuclear membrane for imaging by super-resolution fluorescence microscopy.- Visualizing phosphatidic acid and diacylglycerol at the nuclear envelope in fission yeast.- Measuring molecular mass densities at subcellular resolution using optical diffraction tomography.- Quantification and comparison of protein distribution on nuclear membrane.- Quantifying nuclear shape fluctuations along the cell cycle, with a focus on early mitosis.- Micropost arrays to model ECM fiber obstacles during cell migration in confinement.- Use of nucleoporin-conjugated beads to study the nuclear pore complex assembly on the nuclear membrane.- T4 DNA-induced reconstruction of artificial nuclei in living mouse oocyte.-  Preparation of nucleoplasmic extract and its application in DNA end processing.- Liposome floatation assays to study membrane interactions of nucleoporins.-  Giant unilamellar vesicles (GUVs) to study membrane interaction of nucleoporins.- One-pot reconstitution of GPCRs into unilamellar vesicles for fluorescence-based phospholipid scramblase activity assay.- Identifying genomic DNA sequences near the nuclear lamina using proximity biotinylation with Ascorbate Peroxidase.-  Pairing LaminB1-DamID and Immuno-3D-FISH to resolve and verify peripheral genome organization of adipogenesis.- Measurement of spatial contact map using sequential FISH.

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