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Transformation Génétique de Tissus Embryogènes de Cotonnier by Montes-E

Transformation Génétique de Tissus Embryogènes de Cotonnier

by Montes-E
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Book cover type: Paperback
  • ISBN13: 9783841737496
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Omniscriptum
  • Publisher Imprint: Omniscriptum
  • Publication Date:
  • Pages: 100
  • Original Price: GBP 26.0
  • Language: French
  • Edition: N/A
  • Item Weight: 159 grams
  • BISAC Subject(s): Life Sciences / Biology

Le cotonnier, Gossypium hirsutum L., est la première source de textile végétale. Plus de 40% des cotonniers cultivés sont transgéniques résistants aux insectes ravageurs. La méthode de transgénèse la plus courante est basée sur l'utilisation d'Agrobacterium tumefaciens et sur la régénération de plantes in vitro par embryogenèse somatique. Ce procédé est chronophage, consommateur de main d'oeuvre et de matériel végétal. Cet ouvrage porte sur le développement d'une méthode alternative basée sur l'emploi de tissus embryogènes, faciles à obtenir, comme matériel de départ dans l'espoir de gagner en efficacité et en temps. L'accent a été mis sur sa caractérisation morphologique et histologique, les paramètres de croissance des lignées embryogènes, le type d'agrégat de cellules embryogènes, la phase de culture à laquelle les tissus doivent ètre prélevés pour ètre inoculés, l'influence de l'agrobactérie utilisée. Les paramètres qui ont été définis permettent de proposer un protocole aisé pour transformer génétiquement le cotonnier. Ce livre est l'initiation de toute étude qui voudrait approfondir ces critères et traiter un grand nombre de gènes d'intérèt agronomique grâce ce type de tissus.

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