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Untersuchungen zum Einsatz kurzkohärenter Lichtquellen und numerischer Autofokussierungsverfahren in der digitalholographischen Mikroskopie by Langehanenberg, Patrik

Untersuchungen zum Einsatz kurzkohärenter Lichtquellen und numerischer Autofokussierungsverfahren in der digitalholographischen Mikroskopie

by Patrik Langehanenberg
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Book cover type: Paperback
  • ISBN13: 9783640466955
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Grin Verlag
  • Publisher Imprint: Grin Verlag
  • Publication Date:
  • Pages: 184
  • Original Price: USD 74.5
  • Language: German
  • Edition: N/A
  • Item Weight: 245 grams
  • BISAC Subject(s): Physics / Optics & Light

Doktorarbeit / Dissertation aus dem Jahr 2009 im Fachbereich Physik - Optik, Note: 1, Westfälische Wilhelms-Universität Münster (Centrum für Biomedizinische Optik und Photonik), Sprache: Deutsch, Abstract: Ziel der vorliegenden Arbeit war die Entwicklung von Verfahren zur Rauschminderung, zur Reflexionsebenenselektion und zur numerischen Autofokussierung in der digitalholographischen Mikroskopie. Zunächst wurden die Eigenschaften der eingesetzten kurzkohärenten Superlumineszenzdioden charakterisiert und optimiert. Dabei konnte nachgewiesen werden, dass die Rekonstruktion der hiermit aufgenommenen digitalen Hologramme mit Methoden der räumlich phasenschiebenden Interferometrie möglich ist. Die Bildweite der rekonstruierten Wellenfelder kann anschlie end mittels der faltungsbasierten Propagation variiert werden. Die Untersuchungen ergaben, dass das Phasenrauschen bei Einsatz kurzkohärenter Lichtquellen im Vergleich zur Laserlichtquelle signifikant vermindert wird. Es wurde gezeigt, dass die Anwendung von angepassten Methoden der kurzkohärenten Interferometrie in der digitalholographischen Mikroskopie eine selektive Erfassung und Rekonstruktion definierter Reflexionsebenen ermöglicht. Hierbei ist die axiale Auflösung durch die Kohärenzlänge des verwendeten Lichts bestimmt; eine spektrale Bandpassfilterung des Lichts bewirkt eine definierte Erhöhung der Kohärenzlänge, wodurch die Empfindlichkeit gegenüber Fluktuationen in der Weglängendifferenz, z.B. durch mechanische Instabilitäten, vermindert wird. Da die interferometrisch selektierte Objektebene im Allgemeinen nicht scharf abgebildet wird, wurde durch die Kombination von raumfrequenz- oder gradientenbasierten Bildschärfequantifizierungsverfahren mit der numerischen Propagation ein flexibler Autofokus für die digitalholographische Mikroskopie entwickelt. Hierdurch wird eine nachträgliche automatisierte Fokusstabilisierung sowie eine simultane scharfe Abbildung von Objekten in unterschiedlichen Gegenstandsweiten ermög

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